triple color fluorospot kits (Cellular Technology Ltd)
Structured Review

Triple Color Fluorospot Kits, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/triple+color+fluorospot+kits/Mouse+IFN-%CE%B3+IL-2+Double-Color+FluoroSpot/pmc10081732-98-8-11
Average 96 stars, based on 1 article reviews
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1) Product Images from "Adjuvants Differentially Modulate the Immunogenicity of Lassa Virus Glycoprotein Subunits in Mice"
Article Title: Adjuvants Differentially Modulate the Immunogenicity of Lassa Virus Glycoprotein Subunits in Mice
Journal: Frontiers in tropical diseases
doi: 10.3389/fitd.2022.847598
Figure Legend Snippet: Cytokine secretion measured from mouse splenocytes using a FluoroSpot assay. Geometric mean (GMT) with 95% confidence intervals of (A) IL-2, (B) TNF-α, (C) IFN-γ and (D) polyfunctional cytokine secretion from splenocytes harvested from mouse groups one-week post-dose 2 and stimulated with a LASV GP peptide pool for 24 hours. (n=3 for the ISA 720 groups, n=1-2 for LiteVax groups, and n=3-4 for GPI-0100 groups) A two-way ANOVA, followed by a Tukey’s multiple comparison was used to compare the number of cytokine-secreting cells from immunized groups to the group receiving LASV GP alone, and between different adjuvant formulations of the same antigen concentration, respectively. (*p < 0.05, **p < 0.01).
Techniques Used: Flurospot, Comparison, Adjuvant, Concentration Assay
Related Articles
Flurospot:Article Title: Adjuvants Differentially Modulate the Immunogenicity of Lassa Virus Glycoprotein Subunits in Mice Article Snippet: The neutralization titers (PRNT 50 ) were defined as the serum dilution or mAb concentration that resulted in 50% infectivity reduction using a constrained sigmoidal 4PL nonlinear regression model (GraphPad Software version 9, San Diego, CA). .. The FluoroSpot assay was performed using mouse IFN-γ/TNF-α/IL-2 |